Despite its advantages, this technique has some limitations, such as reduced spatial resolution compared to conventional SEM. Another one is the need for specialized equipment and Sachverständigengutachten to operate the microscope under controlled environmental conditions.
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Samples are generally mounted rigidly on a specimen holder or stub using a conductive adhesive. SEM is used extensively for defect analysis of semiconductor wafers, and manufacturers make instruments that can examine any parte of a 300 logistik semiconductor wafer. Many instruments have chambers that can tilt an object of that size to 45° and provide continuous 360° rotation.[citation needed]
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Rein SEM, cathode is the source of the electron beam. The electron beam is generated by an electron source, typically a tungsten filament or an electron gun. The electron source emits electrons through a process called thermionic emission or field emission, depending on the type of source. The emitted electrons are then accelerated by an electric field and focused into a narrow beam using a series of electromagnetic lenses.
The beam passes through pairs of scanning coils or pairs of deflector plates in the electron column, typically hinein the final lens, which deflect the beam rein the x and Ypsilon axes so that it scans rein a raster fashion over a rectangular area of the sample surface.
Cryo-SEM offers several advantages over conventional SEM for imaging sensitive samples. By preserving the sample hinein its native, hydrated state, cryo-SEM provides more accurate and representative images of the sample's structure and composition. Additionally, cryo-SEM can be used to study dynamic processes, such as the freezing and thawing here of materials, by capturing snapshots of these processes at different stages.
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